The effect of dietary lipid suplementation to Solea senegalensis broodstock, on the quantity and quality of spawns


The aim of this study was to evaluate the influence of dietary supplementation with lipids of sole (Solea senegalensis) broodstock diets on the quantity and quality of spawns. One group (DE group) was fed daily with a diet supplemented with lipids [(6.7% more than control diet (DC group)]. The activities of cathepsins B and D were determined on the viable and nonviable fraction of the spawns from both of groups. The number of spawns and the quantity of eggs was higher for the breeders fed diet DE. The hatching rate was significantly higher (p<0.05) in the group fed with DE diet. Some differences were showed when comparing viable and nonviable eggs. Cathepsin D activities were significantly higher for nonviable fraction when compared with viable eggs. The use of DE diet resulted in a larger number of spawns, suggesting that this diet seems to increase fertility and the hatching rate.



BEIRÃO, J.; SOARES, F.; HERRÁEZ, M.P.; DINIS, M.T.; CABRITA E. 2009 Sperm quality evaluation in Solea senegalensis during the reproductive season at cellular level.Theriogenology, 72(9): 1251–1261.

BAYNES, S.M.; HOWELL, B.R.; BEARD, T.W. 1993 A review of egg production by captive sole, Solea
solea (L.). Aquaculture and Fish Management, 24(2):171–180.

BONISŁAWSKA, M.; FORMICKI, K.;KORZELECKA-ORKISZ, A.; WINNICKI, A.; 2001 Fish egg size variability: biological significance, Ejpau, 4(2): 1-2.

BROMAGE, N. 1995 Broodstock management and seed quality - general considerations. In:
BROMAGE, N. e ROBERTS, R.J. (eds), Broodstock Management and egg and larval quality. Blackwell science. Oxford, UK. 118p.

characterisation of ovarian cathepsin D in the rainbow trout, Oncorhynchus mykiss. Gene,
201(1-2): 45-54.

CABRITA, E.; SOARES, F.; DINIS, M.T. 2006 Characterization of Senegalese sole, Solea
senegalensis, male broodstock in terms of sperm production and quality. Aquaculture, 261(3):

CARNEVALI, O.; CENTONZE, F.; BROOKS, S.;MAROTA, I.; SUMPTER, J.P. 1999 Molecular cloning and expression of ovarian cathepsin D in seabream, Sparus aurata. Biology of Reproduction, 61(3): 785-791.

CARNEVALI, O.; MOSCONI, G.; CAMBI, A.; RIDOLFI, S.; ZANUY, S.; POLZONETTIMAGNI, A.M. 2001 Changes of lysosomal enzyme activities in sea bass (Dicentrarchus labrax) eggs and developing embryos. Aquaculture, 202(3-4): 249–256.

CARNEVALI, O.; CIONNA, C.; TOSTI, L.;LUBZENS, E.; MARADONNA, F. 2006 Role of cathepsins in ovarian follicle growth and maturation. General and Comparative Endocrinology, 146(3): 195–203.

CARNEVALI, O.; CIONNA, C.; TOSTI, L.; CERDA, J.; GIOACCHINI, G. 2008 Changes in cathepsin
gene expression and relative enzymatic activity during gilthead sea bream oogenesis. Molecular
Reproduction and Development, 75(1): 97–104.

EUROPEAN COMMISSION, 2012 Facts and figures on the Common Fisheries Policy: basic statistical data. Facts and figures on the CFP. Fisheries. 48p.

DINIS, M.T.; RIBEIRO, L.; SOARES, F.;SARASQUETE, C. 1999 A review on the cultivation potential of Solea senegalensis in Spain and in Portugal. Aquaculture, 176(1-2): 27-38.

FERNÁNDEZ-PALACIOS, H. e IZQUIERDO, M.S.2009 Efectos de la dieta de los reproductores
sobre la puesta. In: ESTÉVEZ, M.A.C. La reproducción de los peces: aspectos básicos y sus aplicaciones en acuicultura. Madrid. p.338-400.

FU, Y.H.; NISHINAKA, T.; YOKOYAMA, K.; CHIU, R. 1998 A retinoblastoma susceptibility gene
product RB, targeting protease is regulated through the cell cycle. FEBS Letters, 421: 89-93.

HOWELL, B.; PRICKET, R.; CAÑAVATE, P.; MAÑANÓS, E.; DINIS, M.T.; CONCEIÇÃO, L.; VALENTE, L.M.P. 2011 Sole farming: there or thereabouts! A report of the 5th Workshop on the Cultivation of Soles. Aquaculture Europe, 36:42–45.

IZQUIERDO, M.S.; FERNÁNDEZ-PALACIOS, H.;TACON A.G.J. 2001 Effect of broodstock nutrition on reproductive performance of fish. Aquaculture, 197(1-4): 25-42.

KUBTIZA, F. 1999 Nutrição e Alimentação de Tilápias- Parte 2. Panorama da Aquicultura, 9: 41-49.

LACOUE-LABARTHE, T.; LE BIHAN, E.; BORG, D.;KOUETA, N.; BUSTAMANTE, P. 2010 Acid phosphatase and cathepsin activity in cuttlefish (Sepia officinalis) eggs: the effects of Ag, Cd, and
Cu exposure ICES Journal of Marine Science, 67(7):1517-1523.

LAHNSTEINER, F.; SOARES, F.; RIBEIRO, M.; DINIS, M.T. 2008 Egg Quality Determination. In:
CABRITA, E.; ROBLES, V.; HERRÁREZ, M.P.(eds) Teleost Fish Methods In Reproductive Aquaculture: Marine and Freshwater Species. CRC Press (Taylor and Francis). p.149-180.

LUND, I.; STEENFELDT, S.J.; SUHR K.I.; HANSEN,B.W. 2008 A comparison of fatty acid composition and quality aspects of eggs and larvae from cultured and wild broodstock of common sole (Solea solea L.). Aquaculture Nutrition, 14(6): 544–555.

LE FRANÇOIS, N.R.; JOBLING, M.; CARTER, C.;BLIER, P.U.; SAVOIE, A. 2010 Finfish aquaculture
diversification. SPI, Pondicherry, India. 51: 681p.

MORAIS, S.; CANDEIAS MENDES, A.;CASTANHEIRA, M.F.; COUTINHO, J.;BANDARRA, N.; DIAS, J.; CONCEIÇÃO, L.E.C.; POUSÃO-FERREIRA, P. 2014 New for mulated diets for Solea senegalensis broodstock: effects of parental nutrition on biosynthesis of long-chain polyunsaturated fatty acids and performance of early larval stages and juvenile fish. Aquaculture,432: 374-382.

SALZE, G.; TOCHER, D.R.; ROY, W.J.; ROBERTSON, D.R. 2005 Egg quality determinants in cod (Gadus morhua L.): egg performance and lipid in eggs from farmed and wild broodstock. Aquaculture Research, 36(15):1488-1499.

SARASQUETE, J.M.A.C. 2005 Atlas histológico del Lenguado senegalés Solea senegalensis (Kaup, 1858).Consejo Superior de Investigaciones Científicas.Madrid, 18: 184p.

SARGENT, J. 1995 Origins and functions of egg lipids: nutritional implications. In: BROMAGE,
N.R. e ROBERTS, R.J. (eds) Broodstock Management and Egg and Larval Quality. Blackwell Science Ltd., Oxford, U.K. p.353–372.

TINGAUD-SEQUEIRA, A.; CARNEVALI, O.; JOAN CERDÀ, J. 2010 Cathepsin B differential expression and enzyme processing and activity during Fundulus heteroclitus embryogenesis.Comparative Biochemistry and Physiology, Part A,158(2): 221–228.

ULIANA, O.; SILVA, J.H.S.; NETO, J.R.; 2001 Diferentes fontes de lipídios testadas na criação
de larvas de jundiá (Rhamdia quelen), pisces, pimelodidae. Ciência Rural, 31(1): 129-133.

ZAR, J.H., 1996 Biostatistical Analysis. Prentice-Hall, Englewood Cliffs, NJ. 3ª Edição. 718p
How to Cite
SOARES, Florbela et al. The effect of dietary lipid suplementation to Solea senegalensis broodstock, on the quantity and quality of spawns. Boletim do Instituto de Pesca, [S.l.], v. 41, n. 2, p. 397 - 407, july 2018. ISSN 1678-2305. Available at: <>. Date accessed: 13 aug. 2022.
Short Communication